The infectious disease markers for which blood donors are screened include anti–human immunodeficiency virus (HIV), hepatitis B surface antigen (HBsAg), anti–hepatitis C virus (HCV), rapid plasma reagin (RPR) card test for syphilis and malarial parasites.A total of 1000 donors were screened over three years to assess the prevalence of infectious disease markers. Screening for anti–HIV I and II, HBsAg and anti–HCV was carried out by enzyme linked immunosorbent assay (ELISA). Syphilis was tested using RPR card test. Malarial parasite was tested by detection of genus specific plasmodium lactate dehydrogenase.The overall seropositivity for anti–HIV I and II was nine (0.13%), for HBsAg 67 (0.99%), for anti–HCV 13 (0.19%) and for syphilis 42 (0.62%). No sample showed malarial parasites. There was no significant difference (p>0.05) in the seropositivity of various markers between voluntary and replacement donors.