Various studies have been carried out to study fingerprint patterns among β–thalassemia major patients all over India with mixed results. Therefore, an attempt was made to study the association of fingerprint patterns with patients with β–thalassemia major in comparison to controls. This cross–sectional study was conducted in 400 study participants (200 cases & 200 controls) in Department of Anatomy, R.N.T. Medical College & attached group of hospitals, Udaipur to analyse fingerprint patterns among patients of β–thalassemia major. In present study Ulnar Loop (UL) fingerprint pattern was the most common type of fingerprint in cases as similar to controls. However, Whorl (W) type fingerprint pattern was more frequent in cases than controls. Present study also found significant (‘p’<0.001) association between fingerprint patterns and β–thalassemia major.