Fungal infections are common in immunocompromised. Although actual incidence has increased, its real frequency is often underestimated because of difficulty in diagnosis and obtaining proper biological samples. Prospective study was done on 63 cases of immunocompromised patients with fungal infections. The samples were processed, identified and AFST was performed according to CLSI guidelines. Candida species are predominant followed by Aspergillus and Trichosporon species. On AFST, Candida isolates showed high sensitivity to Ketoconazole, Miconazole followed by Clotrimoxazole  and Fluconazole. The MICs of Amphotericin B and Itraconazole for all Aspergillus isolates are within the susceptible ranges. Candida species are common cause of fungal infections in immunocompromised patients followed by Aspergillus species and Trichosporon species. Reporting of antifungal susceptibility in terms of MICs for fungal isolates, helps in prescription of appropriate drug doses by clinician and for early recovery of the patient