Shift work may cause considerable physiological and psychological changes in individuals who are susceptible to circadian rhythm misalignment and sleep wake cycle changes. The present study was conducted in the Department of Physiology, at National Institute of Medical Sciences and Research, Jaipur. 120 cases were included in which 60 healthy non shift workers and 60 healthy shift workers. Comparison between groups Male shift worker, Male non shift worker, Female shift worker, Female non shift worker (MSW, MNSW, FSW and FNSW) was performed using the student’s t–test. Differences was considered significance at p<0.05. Shift work can be stress factor with impact on workers health, workers with shift work schedule load need special attention and frequent health check–up due to higher risk.