Background and Purpose: Antimicrobial resistance in E.coli is of particular concern because it is the most common gram negative pathogen causing infections, particularly urinary tract infections (UTIs) in humans. Due to the production of extended spectrum β–lactamases E.coli exhibits increasing resistance to β–lactams antibiotic

Material & Method^: The study was carried out for a period of 1 year in a tertiary care hospital . 200 E.coli isolates from urine samples of clinically diagnosed urinary tract infection were subjected to antibiotic susceptibility test.ESBL screening test positive isolates were subjected to E test and phenotypic confirmatory test for ESBL production.

Result: : Out of 200 isolates of E.coli 76 (38%) were ESBL producers. ESBL E.coli showed maximum resistance for ampicillin sulbactum (94.7%) followed by co trimoxazole( 84.3%). Resistance to imipenem was 6%.No resistance was noted for colistin .

Conclusion : Knowing the antibiotic susceptibility pattern of ESBL E.coli  will guide the physician in treating UTI caused by ESBL E.coli