Spectrophotometry is one of the branches of spectroscopy where we measure the absoption of light by molecules that are in the gas or vapour state or dissolved molecules/ions. The word spectroscopy is used as a collective term for all the analytical techniques based on interaction of light and matter. Cefoxitin is a second generation cephalosporin with antibacterial activity. Develop and validate stability indicating, simple and accurate method for the determination of Cefoxitin sodium using accurate UV spectroscopic method for the determination of Cefoxitin sodium in powder for injection and perform validation of the method as per ICH guidelines. The standard solution of Cefoxitin sodium was taken and scanned in the range of 200–400nm in the UV– Visible spectrophotometer. From the spectrum obtained the wavelength at which the maximum absorption takes place has been found out. From the spectrum taken, it was found out that the lambda max for Cefoxitin sodium was observed at 231nm. Cefoxitin sodium is very soluble in water, soluble in methanol, sparingly soluble in dimethyl formamide, slightly soluble in acetone, insoluble in ether and in chloroform. The solubility of Cefoxitin sodium was determined by using different aqueous systems like distilled water, 0.1M NaOH, 0.1 M HCL. From the above mentioned solubility studies it was found that the drug has the solubility in water and 0.1 M NaOH.  The solvent selected for this method was 0.1 M NaOH, because in water the drug doesnot show stable lambda max graph. The accuracy of the method was proved by performing recovery studies which were carried out by analyzing the formulation with three different levels like 10%, 20% and 30%. A value closer to 100% indicates that the proposed method is accurate for the analysis.
Thus, it was concluded that the above method was simple, precise and easy to perform and require short time to analyze the drug in the commercial formulations