Our aim is to study the genotypic of uropathogenic Escherichia Coli strains isolated from patients with urinary tract infection by using PCR methods. Urinary tract infection is one of the most common infections among male and female of all age groups affecting 150 million people worldwide. This study was carried out in the Department of Microbiology, Meenakshi Medical College Hospital and Research Institute, Kanchipuram from March  2017 to March 2018. Totally 500 urine samples were collected and among these 280 were culture positive, out of which females were 221 and male were 59 of varying age groups between 0 – 70 yrs of age. Out of 500 urine samples collected 280 were culture positive. Among them 221 were females and 59 were males. E.Coli was significantly present in culture compared with other Bacteria. This study emphasis the fact that genotypic pattern of UTI can guide the clinicians in choosing the right antibiotics for treatment so that misuse of antibiotics may be prevented