Needlestick injury (NSI) is a commonly encountered occupational hazard which may transmit various blood–borne infections. In the Kingdom of Saudi Arabia (KSA), the main risk factor is the low awareness about injection safety measures. This study aims to assess the level of knowledge, attitude and practice of injection safety as wel as to measure the prevalence of NSI. It includes (250) nurses in Primary Health Care Centers(PHCCs) belonging to Ministry of Health in Al–Ahsa city, KSA from January 2018 to March 2018. Self–administered questionnaire was used for data collection. Overall 54.8% of the nurses had a high level knowledge. High attitude and high practice was observed among 44.8% and 68.4% respectively. The prevalence of NSI was 40% .

     Knowledge of nurses working in PHCCs in Al–Ahsa regarding safe injection is quite acceptable. Despite that, the prevalence of NSI is relatively high with a low attitude towards reporting to responsible authorities.