Staphylococci are normal inhabitants of human skin and mucous membrane. They play important role as human pathogens causing both nosocomial as well as community acquired infection. Over the time there has been increased prevalence of MRSA among hospitalized patients resulting in increased morbidity and mortality among patients. AIM & OBJECTIVE of the study is to detect the distribution of Staphylococcus in various clinical specimens and to detect antibiotic susceptibility pattern of these strains against various antibiotics used for the treatment of patients. MATERIAL & METHOD– Staphylococcus strains were identified using standard procedures and antibiotic susceptibility testing was done on Mueller Hinton agar by Kirby Bauer disc diffusion method. RESULT–Total sample received for culture & sensitivity over 6 months from May to October 2018 were 3452 of which 1054 (36.32%) were sterile and 2198 (63.67%) showed growth. From 2198 (63.67%) samples 350 (15.92%) showed growth of Staphylococcus species. From these Staphylococcus strain MRSA were 190 (54.28%) followed by MRCONS 86 (24.57%), MSSA 50 (14.28%) and MSCONS 24 (6.85%) Most of the staphylococcus aureus strains isolated were from pus and wound swab 236 (67.42%) followed by blood 64 (18.28%), urine 16 (4.57%), vaginal swab 14 (4%), peritoneal fluid 8 (2.28%), sputum 6 (1.71%) and others 6 (1.71%). Male 194 (55.42%) were more commonly infected with Staphylococcus aureus then females 156 (44.57%).Most common age group affected was between 20 to 30 years  accounted to 90 (25.71%) cases followed by 30 to 40 years 75 (21.42%), 40 to 50 years 60 (17.14%), 10 to 20 years 52 (14.85%), more than 50 years 40 (11.42%) cases, least number of cases were reported from age group less than 10 years 33 (9.42%). Majority of Staphylococcus aureus strains isolated were from hospitalized patients of Surgery department 132 (37.71%) followed by Obstetrics and Gynecology 60 (17.14%), OPD 56 (16%), Pediatrics 42 (12%), ENT 20 (5.71%), Medicine 20 (5.71%), TICU 14 (4%), Orthopedics 06 (1.71%). Antibiotic susceptibility pattern of Staphylococcus strains showed 100% sensitivity to Vancomycin, followed by Gentamicin 81.80%, Ciprofloxacin 50.28%, Erythromycin 35.42%, Clindamycin 34.28%. Maximum resistance was noted for Cefoxitin 78.85%.