A Fourier transform infrared (FT–IR) spectrometric method was developed for the rapid, direct determination of Prednisolone in different Ayurvedic,Siddha and Naturopathic Formulations. Prednisolone is widely used in Ayurvedic preparations for the quick relief of pain in joints and arthritis. But on long term it can result in steroidal toxicity. The Universal ATR spectra  of Prednisolone  with a specific peak area at 1185.4–939.19 cm^–1 were recorded and used for this study. Quant Builder, an OPUS software was used for data processing. An average recovery of 98.2% of Prednisolone from different dosage form with a correlation coefficient of 0.985 was obtained. The linear regression equation for Prednisolone was calculated to be y=–18.491–11.206*x. The method had excellent reproducibility for the standard of 40 mg, 38.58 ±1.02 mg (n =6). The method gave rise to linear data in the range 2–50 mg with accuracy and precision in the range 0.56–2.4%. Therefore, this FT–IR–spectrophotometric assay was accurate, and may be recommended for the simple quantification of Prednisolone in Ayurvedic, Sddha and naturopathic preparations