Sleep deprivation is becoming increasingly common in today‘s society because of adopting a 24/7 lifestyle. It causes alteration in circadian rhythm and imposes great social, psychological, economic and health related problems. This study was designed to explore the effect of, zolpidem on certain behavioural parameters namely (1) body weight (2) Locomotor activity and (3) anxiety level to stress, produced as a result of sleep deprivation .Healthy male albino mice weighing between 25–30 grams were divided into three groups with six animals in each group. The first and second groups were treated as naïve (without sleep deprivation) and 48 hours sleep deprived (by placing on a grid suspended over water, based on modified method of Shinomiya et al.) respectively. The study group third was sleep deprived and administered zolpidem to strength of 0.02mg/ml, by dissolving 5mg tab in 250ml of gum acacia orally once daily for 24 days.The change in behavioural effect after administration of zolpidem was evaluated by measuring locomotor activity by actophotometer and anxiety level by Elevated plus maze model. The effect of zolpidem in sleep deprived albino mice shows beneficial role on the behaviour parameters in sleep deprived albino mice.