Introduction :– Leptospirosis is caused by infection with pathogenic spirochaetes of the genus Leptospira. It could be diagnosed with the help of Dark ground microscopy, in– vitro culture, serology and molecular method, each one with its merits and de– merits.

Aims & Objectives :– To find out the prevalence of leptospiral infection in patients of pyrexia of unknown origin, jaundice, by immunological and molecular techniques

Materials & Method:– In this study 110 urban – rural patients with history of pyrexia of unknown origin or jaundice were selected.

Conclusion:– In serological tests IgM positivity was found in 7 cases in pyrexia with jaundice group. Leptospira serovar autumnalis was found to be the most common that is 13 case, followed by serovar canicola 3 cases and icterohaemorrhagiae in 1 case