Context and rationale: Formulating good quality of multiple choice questions (MCQs) is a demanding task. Difficulty index (DIF) and discrimination index (DI) are use to determine the quality of the MCQs.

Aim of the study: To assess the quality of the MCQs used in Anatomy examination.

 Methods: Study was conducted on ninety (90) 1^st year MBBS students of Enam medical College, Savar, Dhaka, who appeared in second term Anatomy examination, September 2017. It comprised of twenty (20) Anatomy multiple “true/false response” type MCQs with no negative marking. Difficulty index (DIF I) and discrimination index (DI) of each item was analyzed.

Results and conclusions:  Maximum 11(55%) item have the DIF within the acceptable range (P =30–70%), and 1 (5%) item was too difficult (P < 30%). Whereas DI of majority 11 (55%) items was excellent (d > 0.35) and one third 5 (25%) of the items was poor (d < 0.20). Item analysis is an important tool to discriminate good and bad test items. It helps to increase the need of skills in constructions of items and identifies the specific area which need more focus to discard or clarity