Introduction: Hepatitis B Virus (HBV) infections are a global public health problem. India falls in the intermediate endemicity zone with prevalence of 2–7%, with an average of 4%. Timely diagnosis of Hepatitis B provides individuals an opportunity for intervention and to reduce transmission. 

Aim: To compare sensitivity and specificity of rapid diagnostic test with ELISA.

Material and Methods: A study was conducted in the Department of Microbiology at Dr Ram Manohar Lohia Hospital from January 2018 to June 2018. Blood samples were tested for HBsAg by ELISA and positive and negative samples were also tested with rapid diagnostic test.

Results: In our study 13964 blood samples were tested for HBV using ELISA. Sensitivity and specificity of the rapid card test was found to be 80.15% and 100%respectively.

Discussion: Rapid diagnostic test can be used during emergency or odd hours, but their results must be followed by ELISA test results in a tertiary care hospital. Care must be taken to minimise false negative result to avoid silent transmission of infection.