Introduction: The behaviour and attitude of health providers, especially nursing students, towards oral health affect their capacity to deliver oral health care services. Aim: The purpose of this study is to evaluate the oral health attitude and behaviour among nursing students in Ahmedabad, India and to find any gender‑based differences in terms of oral health attitude and behaviour.  Materials and Methods: A total of 200 (50 students per year) nursing students of Gujarat Institute of Nursing Education and Research, Ahmedabad, India interviewed through a questionnaire included in the study. Results: Statistical significance was observed for the responses between the students from different academic years to visit dentist office, dentist visit on toothache, brushing frequency, careful brushing of teeth, use of floss and mouthwash, careful self‑evaluation of brushing and satisfied appearance of teeth and gums. Male students preferred to use floss than female students. With regards to worrying about bad breath, male students had more “yes” responses than females.  Conclusions: Oral health attitude and behaviour increased with the increasing levels of education, and no significant differences were observed among male and female nursing students.