Osteomyelitis is one of the most important causes of morbidity in our country. Advances in the identification of infections and early diagnosis of Osteomyelitis have led to the improved management of Osteomyelitis. This study was undertaken to determine the bacteriological profile of Osteomyelitis and the antibiotic resistance pattern of various isolates obtained as it is an important cause of morbidity. So, appropriate drug selected by antibiotic sensitivity testing should be used to treat Osteomyelitis.