IJSR International Journal of Scientific Research 2277 - 8179 Indian Society for Health and Advanced Research ijsr-8-2-17894 Original Research Paper blaNDM-1 GENE CARRIAGE IN THE ICU OF TERTIARY CARE HOSPITAL IN NORTH INDIA Anuj Sud Dr. Dr. Beena Uppal Dr. February 2019 8 2 01 02 ABSTRACT

 BACKGROUND:– New Delhi metallo–β–lactamase 1 (NDM–1) enzyme leads to multidrug resistance and has been detected from bacteria in many countries. The study was done To detect bacteria carrying blaNDM–1 gene from intensive care unit (ICU) patients and correlate the change with increasing duration of ICU stay.

METHODS:– Blood and urine samples were collected from 140 patients thrice– 0–2 days, 3–7 days and ˃7 days of ICU admission. All bacterial isolates resistant to meropenem were evaluated for Metallo–β–lactamase (MBL) production. blaNDM–1 gene was detected using Real Time PCR from the MBL producers.

RESULT:–  Overall, 458 samples (229 each) of blood and urine were collected and 75 gram negative bacteria were isolated. From these, 46.7% (35/75) strains were found to be carbapenem resistant and blaNDM–1 gene was detected in 17.3% (13/75).

CONCLUSION:– High prevalence of bacteria carrying blaNDM–1 gene was seen in ICU patients increasing the burden on healthcare