PURPOSE: Pyogenic wound infection is major cause of morbidity. Hence it is mandatory for every pus samples to undergo culture and sensitivity. To Identify and isolate aerobic bacteria from pus samples and to study antibiotic profile of isolate.

OBJECTIVES: 1.To know the percentage of aerobic bacteria from the pus samples sent to the laboratory.

2. To study antibiotic susceptibility profile of isolates

METHODOLOGY: The Study Was Conducted in the Department of Microbiology, Gandhi Hospital, Secunderabad. Total of 1372 Pus samples send from different departments from January2016 to November 2016 were included in the study. Culture & Sensitivity Was Performed. Antimicrobial Profiling of isolated strains was determined by Kirby–Bauer Disc–Diffusion Method.

RESULTS AND DISCUSSION: In our study among 1372  pus samples ,442 (32%)were found to be positive for   aerobic culture , Staphylococcus  aureus –124 isolate (28%) is the most common isolate followed by Klebsiella pneumoniae–115 isolates (26%) , Escherichia coli–86 isolates (19%) , Pseudomonas aeruginosa –40 isolates (9%), Coagulase negative Staphylococcus–27 isolates (6%)  Proteus mirabilis–2 isolates 4 (5%),  Proteus vulgaris–17 isolates (4%) ,Streptococcus  pyogenes (groupA)–10 isolates (2%) . Among Gram positive organisms Amikacin ,Gentamicin  levofloxacin , linezolid, tigecyclin, teicoplanin and vancomycin were susceptible drugs and were resistant to ampicillin while among Gram negative organisms amikacin,  imipenem ,amoxiclav, azithromycin, pipercillin +tazobactum were found to be susceptible  and resistant to ampicillin,amoxicillin

CONCLUSIONS: Changing antimicrobial resistances pose challenge in treating pyogenic infections. Appropriate and judicious selection of antibiotics would limit emerging drug resistant strains in future to treat these clinical conditions successfully