Aim: Aim of this study was to compare the effectiveness of 1% Peracetic acid, 2% Chlorhexidine Gluconate and Pancha Tulsi solutions to disinfect gutta–percha cones.

Methodology: 110 gutta–percha cones of size 70 were used and divided depending upon the type of disinfecting agents utilized. Cones used in the study were contaminated with E. faecalis and then immersed in 1% Peracetic acid, 2% Chlorhexidine Gluconate and Pancha Tulsi for 1 and 5 minutes. For analysing surface topography, cones were immersed in disinfecting solutions and observed under scanning electron microscope.

Results: 1% Peracetic acid showed best results for both 1 and 5 minutes’ disinfection and there was statistically significant difference (p<0.01) between the test groups for 1 minute. Scanning Electron Microscope analysis showed surface changes in all the groups.

Conclusion: All the solutions tested were effective in disinfection of the cones. However, 1% Peracetic acid showed rapid disinfection potential when compared with the above test groups.