Diffuse large B cell lymphoma represents a very heterogeneous group of tumors and different subtypes have been identified by genetic testing. IHC algorithms have been identified as a surrogate to genetic testing, by classifying DLBCL cases into Germinal centre group (GCB) and Activated B cell (ABC) group. Identification of c MYC expression and double expressor lymphoma (DEL) have been shown to correlate with poor prognosis in DLBCL. Present study aim at classifying DLBCL according to HANS algorithm and identifying c MYC expression and DEL by applying different immune markers with the help of IHC.

MATERIAL AND METHODS. A prospective study of 50 cases of biopsy proven nodal DLBCL was conducted. Different immune markers were applied as per the required IHC panel of DLBCL, cases were classified into prognostic subtypes, and c MYC expression and DEL groups were identified.

RESULTS– out of 50 cases of nodal DLBCL, 18% were of GCB and 82% were of ABC group. c MYC expression was observed in 40% cases whereas DEL cases were 16%.

CONCLUSION– DLBCL cases should be classified into prognostic sub types by applying immunohistochemical algorithms and study of expression of adverse prognostic markers should be done by IHC to plan better treatment for these patients.