Background : To describe findings in vitreous fluid samples in routine cytology practice. Methods: This is a retrospective  review of a series of 56 patients having a total of 56 vitreous fluid cytology determinations. Results : Twenty Nine of the 56 patients (51.7%} have cytologic results highly suggestive of a specific disease process. These include one case of malignant small round cell tumor, one case of fungal endophthalmitis; one case of bacterial endoopthalmitis. There are no vitreous fluid cytology determinations falsely positive or falsely negative for malignancy in this series. Neutrophils, lymphocytes, and histiocytes were the predominant vitreous fluid cell type in the case of an inflammatory process. Conclusion: The cytologic examination of vitreous fluid has been shown to be a valuable modality for diagnosing intraocular disease.