Background:. In tropical countries,  the extent of sun exposure , skin pigmentation play an important role altering the vitamin D levels and hence the causation of uterine fibroid in Indian women.

Aim and objective:  To determine  association between serum vitamin D, calcium and Phosphate levels with uterine fibroid in  premenopausal women.

Materials and Methods: Forty women with uterine fibroid within the age group of 25 to 45yrs were enrolled as cases with forty healthy control. All participants were  evaluated on the basis of a questionnaire, serum 25 hydroxyl vitamin D  ,calcium and  phosphate.

Results:  Most of the cases were home makers with sun exposure less than 1 hr/day. Fibroid uterus was commonly associated with multi–parity without history of oral contraceptive pills. There was a significant fall in serum Vitamin D as well as calcium levels (p<0.05) in cases  with  significant negative  association between vitamin D  and  the size of fibroid.

Conclusion: Vitamin D and Calcium  have a  strong association with  uterine fibroid hence  could be treated as  risk factors for developing advanced stage symptomatic fibroid uterus.