Gallstones if present within the gall bladder or biliary tree is often complicated by cholangitis, which ranges from mild to severe life threatening conditions. This study was conducted with an aim to evaluate the microbiological profile of bile from gall bladder in patients undergoing cholecystectomy and to study their antibiotic sensitivity pattern. A total of 40 bile samples were cultured, out of which 20 samples (50%) were found to be culture positive. Most common organism isolated from the culture was Klebsiella pneumoniae (30%), followed by Escherichia coli (25%), Pseudomonas spp. (20%), Acinetobacter spp (10%) and others. Almost all the isolates were sensitive to higher antibiotics like colistin and tigecycline, with varying degree of sensitivity against other antibiotics.