The biosynthesis of nanoparticles has become an area of great interest in recent years due to a growing need to develop environmentally friendly technology in material synthesis. In this study our aim was to investigate a possible nanoscience mechanism of action of homoeopathic medicine. Homeopathic medicines are prepared through a characteristic process known as potentization. Homoeopathic mother tincture   Equisetum hymale is capable of  precipitating silver nano particles from silver nitrate  at ambient temperature . In recent years UTI in females has been the one among the most recorded cases in India, Urinary tract infections are common in women, and many women experience more than one infection during their lifetime . The nanoparticles were characterised   by UV spectrophotometer , were the peaks obtained are 250 for mother tincture, 217 for 200C, 203 for 15C, 201.6 for 3OC, 222.6 for 30C (self potentised) respectively. AFM shows the shape and size  of particles and by FTIR the functional groups in the mother tincture . The synthesized silver nanoparticles were generally found to be effective as antimicrobial agent against some important human pathogens like E–coli, Pseudomonas , Klebsiella , Staphylococcus aureus which cause disease