IJSR International Journal of Scientific Research 2277 - 8179 Indian Society for Health and Advanced Research ijsr-8-3-18226 Original Research Paper In vitro biofilm formation by uropathogenic Escherichia coli and its pattern of antimicrobial susceptibility Goyal Dr. March 2019 8 3 01 02 ABSTRACT

 

Background–

 The most common cause of urinary tract infection is Escherichia coli, though other bacteria or fungi may rarely be the cause. Recurrent UTI’s that are predominantly caused by uropathogenic Escherichia coli’s forms biofilm.

Objectives–

1. To determine the biofilm formation by E.coli.

2. To evaluate the antibiotic susceptibility testing.

 

Material and methods–

The present prospective study was carried out from December 2014 to May 2015 in the Department of Microbiology of SMS medical college, Jaipur. A total of 100 consecutive, nonrepetitive E. coli isolates were subjected to biofilm formation study by Christensen’s tube adherence method. All the isolates were also subjected to antimicrobial susceptibility testing by Kirby‑Bauer disc diffusion method in accordance with the Clinical Laboratory Standard Institute 2013) guidelines and followed inclusion and exclusion criteria. Epi–info software was used for data analysis.

 

Results–

Out of the 100 E. coli isolates studied, 62 (62%) were positive for biofilm formation. Overall drug

resistance was found to be very high among both biofilm as well as non–biofilm forming isolates indicating excessive drug resistance among both community and hospital organisms.

 

 

Conclusion–

The results of present study indicated a need for continued surveillance of antimicrobial resistance and greater understanding of the nature of biofilm producing uropathogens causing UTI, so as to increase positive outcomes of clinical interventions and the development of novel and more effective treatments for the problematic diseases.