Background: Megaloblastic anaemia is very common in India, but there is lack of data pertaining its prevalence and various conditions causing it . We did a reterospective study to facilitate documentation of data for the benefit of patients sufferin from megaloblastic anaemia. Materials and Methods This is a 5 years retrospective hospital based study. A total no. of 21150 patients over a period of 5 years i.e. from Jan. 2014 to Dec. 2018 in the central pathplogy lab ,department of Pathology, G.R. Medical College and associated Jaya Arogya hospital, Gwalior were examined . Results: The mean age of presentation was 24.43. The male : female ratio was 1:1.25. Out of 21150 subjects, 3883 (18.36%) were found to have megaloblastic anaemia. Prevalence of megaloblastic anaemia in present study is 18.36%. Conclusion: Despite a few limitations our data represents comprehensively the prevalence of megaloblastic anaemia. Pattern & distribution of Megaloblastic anemia is of extreme diagnostic importance for the benefit of patient.