India is a country with rich traditional medicine knowledge and is a frontier to the entire world with a larger documentation of medicinal plants. The herbs and their medicinal uses attract a large number of researchers globally. A larger population of India relies heavily on the herbal medicines for their primary health care needs. Medicinal plants play a key role in achieving this object of WHO. Pharmaceutical research mainly focuses on the screening of active constituents of plants and their isolation. Both WHO and AYUSH encourage and design the quality analysis of herbal raw materials and finished products in order to ensure their quality and safety. The main objective of the present study was to determine the phytochemical profile in the selected polyherbal formulation Dermoshine (DSE) by using GC–MS technique. Totally 16 compounds were identified and safety parameters such as microbial load and heavy metals are found within the limits. Other parameters like pesticide residues and aflatoxins are found below detectable limits. The results give a positive indication on the safety and quality of DSE. Further, study is focused on the other advanced instrumentation like LC–MS analysis, Toxicological and Pharmacological studies are under process.