Background: Diabetes in pregnancy or Gestational Diabetes Mellitus (GDM) is defined as carbohydrate intolerance that begins or is first recognized during pregnancy. GDM is a prevalent and potentially serious condition both for mothers and neonates. The objective of this study was to assess the role of glycated hemoglobin A1c (HbA1c) level in assessing gestational diabetes (GDM). Materials & Methods– A total number of 111 participants, (61 cases and 50 controls) attending ANC clinic of a tertiary care hospital, were recruited. Data on sociodemographic and clinical correlates were collected and fasting and post prandial Blood glucose, and HbA1c estimation was done was done in all participants. Results– In present study it is observed that HbA1c levels in GDM patients was significantly more than that those of normal pregnancy (6.30±0.587% verses 4.22±0.616%). (p=<0.001). Conclusion: The results show that HbA1c, in combination with OGTT, may be a useful screening tool for GDM.