In the present study nephroprotective proprietary polyherbal formulation Urizone was chosen for screening the phytochemical constituents by qualitative and quantitative methods. The results indicates hydro–alcoholic extract of Urizone contains alkaloids, flavonoids, terpenoids, carbohydrates, protein and amino acids. Quantitative analysis were also conducted to determine the amount of primary and secondary metabolites present in the hydroalcoholic extract of Urizone. The amount of primary metabolites such as Total carbohydrates 11.10 %, total proteins 2.05 % and total lipids was found 0.17 %. The amount of secondary metabolites found in the hydroalcoholic extract are Alkaloid content was found 79.68 mg/gm, Phenoilc content was 282 mg/gm, tannin content was 125.5 mg/gm, flavonoid content was 0.784 mg/gm, Phytosterols was 0.11 mg/gm and Saponins 0.245 mg/gm. The results concludes that the formulation is potential source of various phytoconstituents.