Despite considerable efforts directed towards reducing transmissible pathogens, transfusion transmitted bacterial infection remains one of the common cause of complications associated with transfusion. Reports from studies conducted in various countries are showing a high incidence of bacterial contamination of donor blood. There are no studies indicating the prevalence of bacterial contamination of blood and its components in Andhra Pradesh. In the present study an analysis of blood and blood component microbiological cultures in our tertiary care referral teaching hospital blood bank from January, 2015 to July ,2018 was performed. A Total of 652 blood and blood component samples were submitted for bacterial and fungal culture. Out of which 20 (3.0%) samples were found to be positive for different bacterial agents. The probable cause for contamination may be due to cutaneous bacteria followed by asymptomatic bacteremia in donors.