Decalcification is a process of complete removal of calcium salt from mineralized tissues like bone and teeth and other calcified tissues. The physical hardness, which is, a unique characteristic of these tissues makes it necessary to “soften” them by removing the mineralized component. Human dentition and bone has been the subject of intense histological investigations for many years. Demineralization or decalcification of tissues is a routine process carried out in most laboratories by the use of various acids or chelating agents.In the manual method of decalcification, hard tissues are placed in a decalcifying agent at room temperature with changes of the solution at regular intervals till the end point is reached. Aim: The aim of the study is to evaluate commonly used demineralizing agents & to identify the best decalcifying agent.Materials and Method : The present study included decalcifying solutions: 10% formal nitric acid, 8% formal nitric acid, 10% nitric acid. Sixty (n=60) natural teeth (twenty in each group) will be decalcified in each of the decalcifying solutions and subjected to chemical end‑point test. The decalcified teeth will be then routinely processed, sectioned, and stained with hematoxylin and eosin stains. Statistical Analysis: One way ANOVA will be used and compartive analysis by chi square test.Result: Considering preservation and staining of both hard and soft tissues, superior results have been obtained by 10% HNO3, followed by 8% Formal nitric acid and 10% formal nitric acid