Diabetes is defined as disturbance in intermediary metabolism manifesting as chronic sustained hyperglycemia, primarily due to either an absolute or relative lack of insulin. During the last 2 decades research and data is showing that vitamin D3 deficiency could be a risk factor in many chronic diseases like Diabetes mellitus, Hypertension, Dyslipidemia Cardiovascular diseases, Auto immune disorders, Tuberculosis and some cancers. Many studies have demonstrated an inverse relationship between vit D3 levels and Diabetes mellitus. Studies in India have demonstrated that the level of vitD3 in the population is low and there is high prevalence of chronic diseases like Diabetes, Hypertension and cardiovascular disease among vit D3 deficient subjects. However there is a paucity of literature regarding the levels of Vit D3 in type2 DM, which is common in our community. In view of the above facts that the present study has been undertaken. Methods: Cross sectional study. Cases of type2 Diabetes Mellitus admitted in the wards of Government General Hospital , Kakinada diagnosed as per the ADA criteria, between ages of 35–75 y ears (inclusion criteria). Results: VitD3 was found to be significantly lower in the study group (19.91 +/– 7.0) as compared to the control group (32.22 +/– 4.0). Conclusions: Significant Vitamin D deficiency was present in Diabetic patients compared to healthy subjects.