Enterococcus faecalis is one of the most prevalent pathogen isolated from root canals of teeth where previous treatment has failed. Thorough debridement of root canal space is a significant objective for complete reduction of micro–organisms. Intracanal irrigants have been used as an adjunct to intensify antimicrobial effect of cleaning and shaping in endodontics. The antimicrobial efficacy of herbal extracts as endodontic irrigants is evaluated and compared with the standard irrigant sodium hypochlorite and chlorhexidine against clinical strains of enterococcus faecalis. The antimicrobial assay was determined by measuring the mean zone of inhibition (in mm) by agar well diffusion method. Results showed that chlorhexidine and sodium hypochlorite showed the maximum anti–bacterial efficacy. Acacia catechu showed significant anti–microbial activity whereas betel vine showed the least. Thus, it can be used as a probable root canal irrigant for prevention of re infection.