Asymptomatic bacteriuria or asymptomatic UTI is the isolation of a specified quantitative count of bacteria in an appropriately collected urine specimen obtained from a person without symptoms or signs of urinary infection. (1) This study was undertaken to find out prevalence , causative agents, and antimicrobial sensitivity pattern of asymptomatic bacteriuria (ASB) in pregnant women attending our center. Total 300 asymptomatic pregnant women were screened during April 2018 to September 2018 . Isolates were identified by conventional methods and one Streptococcus sp. was identified by the Vitek 2 Compact system. Asymptomatic bacteriuria were more common in primigravidae & during 3rd trimester of pregnancy. E.coli was the commonest organism isolated (38.23%) followed by Coagulase negative Staphylococcus (CONS) (23.52%), Klebsiella pneumoniae (17.64%) and Enterococcus sp. (17.64%).Gram negative isolates were resistant to most of the common oral antibiotics. Hence, all pregnant women should be routinely screened for asymptomatic bacteriuria during antenatal check–up .