Introduction: Vitamin D deficiency is highly prevalent in India due to lifestyle and environmental factors. This "pandemic" of vitamin D deficiency is of concern because of its association with cardiovascular, musculoskeletal, infectious, autoimmune diseases but the relationship between ischemic stroke and Vitamin D and PTH levels is still not conclusive. Aims and objectives: We planned a cross sectional case control study to determine the association of vitamin D deficiency with ischemic stroke. Materials and Methods: In the present study 50 patients were included who were diagnosed with ischemic stroke. The inclusion criteria were: i) Presentation within 7 days of onset of ischemic stroke ii) Prestroke Modified Rankin Score (MRS) < 2 iii) Age ≥ 45 years. Patients on vitamin D and calcium supplementation, those with renal and hepatic impairment and those who underwent thrombolysis were excluded. All patients had a CT or MRI brain scan at baseline. A total of 50 age– and gender–matched healthy controls were included. Results and conclusion: We found that there was no significant difference in levels of 25(OH) D levels (p = 0.75) and iPTH levels (p = 0.10) between cases and controls.