Thyroid gland is the largest and highly vascular endocrine gland situated in the anterior part of lower neck. It is a H shaped gland with two lateral lobes connected by a narrow isthmus. A wide range of variations have been reported in both its morphology and development. We report a case of incidental intraoperative finding of isthmus agenesis from KATURI MEDICAL COLLEGE, Chinakondrupadu, Andhra Pradesh.