Antibody response against Dengue virus – infection with dengue virus induces the production of both neutralizing and non neutralizing antibodies. The neutralizing antibodies are protective in nature. Such antibodies are produced against the infective serotype as well as against other serotype. Hence, protection to infective serotype stays lifelong but cross protection to other serotypes diminishes over few months. The non neutralizing antibodies last lifelong and are heterotypic in nature. They are produced against other serotypes but not against the infective serotype. Such antibodies produced following the first serotype infection can bind to a second serotype, but instead of neutralizing the second serotype, it protects it from host immune system by inhibiting the bystander B cell activation antigen against the second serotype. The above phenomenon is called Antibody Dependent Enhancement which explains the reason behind the severity of secondary dengue infection. This prospective study was conducted to assess the pathophysiologic and prognostic role of proinflammatory and regulatory cytokines in the inpatient population tested positive for Dengue fever by using commercially prepared ELISA kits for IFN–gamma. A total of 100 blood samples for this study were collected from pediatric patients and adult patients. The patients were tested for NS1 antigen of DENV and IgM antibodies against DENV. The levels of Positive cases were then selected for the study as per their platelet count. IFN–gamma was obtained using ELISA and statistically analyzed and results were interpreted. One third of the dengue positive patients had very high levels of IFN–gamma level. IFN–gamma values and clinical features were analyzed using Pearson’s Chi Square Test and the Significance was calculated. There was a significant association (p=0.025) between the IFN–gamma categorization and the clinical features i.e. patients with Severe Dengue or Dengue with danger signs had significantly higher number of IFN–gamma levels. As increased IFN–gamma has been related to diminished viral clearance from the host, disease severity might also be related to its serum level.