Purpose to study the prevalence of chronic dacryocystitis in relation to age , sex with the most prevalent organisms and their antibiotic sensitivity along with the correlation, if any, between nasal, conjunctival and lacrimal isolates ranged from 14–65 years. Cross sectional study of 100 patients with chronic dacryocystitis were selected. ROPLAS and lacrimal syringing was done. Swabs from nasal,conjunctival, lacrimal fluid were taken and gram stain and culture sensitivity was done, data statistically analysed and we found that the most common age group with chronic dacryocystitis was 31–41 years. In lacrimal swab gram staining, gram positive organisms were more common with Staphylococcus aureus as the most common organism and sensitive to Tobramycin, Ceforoxime, Ceftriazone, Amoxycillin and Gatifloxacin. . The most prevalent organism isolated from the nasal and conjunctival culture was Staphylococcus aureus .While, no significant relation was found between nasal, conjunctival and lacrimal flora