Tuberculosis control can significantly be increased if more effort is given to improve knowledge and attitudes of physicians towards prevention and control of disease. This study aimed to assess the knowledge about recent change in RNTCP guideline (2016) for diagnosis and treatment of tuberculosis among medical students and interns. Sample size was 295, including students of 3rd Professional MBBS part 1, 3rd Professional MBBS part 2 and Interns of a Medical college in West Bengal. The study was done with the help of specially prepared questionnaires. Mean scores of different batches did not show any significant difference . mean score of 3rd Prof part 1 students was best. Overall response was not satisfactory as many questions had more than 50 percent wrong responses. 3rd Prof MBBS part 1 students , probably after passing microbiology and pharmacology in 2nd Prof MBBS examination fared better in the questionnaire than their senior fellows.