Introduction: Abnormal vaginal discharge is most common complaint in female patients attending gynaecology outpatient department. It is most commonly caused due to bacterial vaginosis, vulvovaginal candidiasis and trichomoniasis. These infections also enhance the chance of acquisition and transmission of a range of sexually transmitted infections including HIV by two to five times. This study was conducted to analyse the prevalence of causative organisms of vaginal discharge in patients attending gynaecology OPD and in high risk groups attending STI clinic from January 2016 to December 2017. Aims and Objectives: • To determine the prevalence of common causes of vaginal discharge. Materials and method: • Retrospective study was carried out in RSTRRL, Department of Microbiology, TNMC & BYL Nair Ch. Hospital, funded by NACO. • Gram staining for Nugent scoring and wet mount of the vaginal swabs was done. • Culture and sensitivity was done for the Neisseria gonorrhoeae. • VDRL, TPHA and HBV, HCV Antibody ELISA, ELISA for Chlamydia trachomatis were done as per National guidelines. Results: In the time period total 2139 swabs were collected. On analysis maximum number (82%) of patients were from 25–44 years of age group. Prevalence of Bacterial vaginosis were found to be 53.30%, non– gonococcal cervicitis 33.90%, and Candidiasis 19.58%, Chlamydia 3.30%. Neisseria gonorrhoeae was isolated in 3 patients. Conclusion: This study emphasizes the role of laboratory in etiological diagnosis in patients with vaginal discharge. There is a need for creating community awareness about health–care facilities and self–concern in women for their own health needs. Regular screening should be made mandatory.