BACKGROUND: According to World Health Organization (WHO) lymphomas are classified into Hodgkin’s lymphoma and Non–Hodgkin lymphoma. Non–Hodgkin lymphoma is further subclassified based on the stage of maturation and cell of origin. MATERIALS AND METHODS: The immunohistochemical profile of nodal Non–Hodgkin lymphoma (NHL), using CD3, CD5 (T cell lineage), CD19, CD20 (B cell lineage) CD10, CD23, CD30, CD43, CD79a, Bcl2, Bcl6, ALK, EMA and Cyclin D1 was done to categorize and validate nodal NHL. RESULTS: Morphological diagnosis on haematoxylin and eosin staining of 41 cases of nodal NHL, 31 were diffuse type, five small lymphocytic lymphomas and five follicular lymphomas. After IHC, 30 cases were DLBCL, four follicular lymphomas, three small lymphocytic lymphomas, three mantle cell lymphomas and one peripheral T cell lymphoma not otherwise specified. CONCLUSION: Lymphomas can be diagnosed on morphology, and with the application of monoclonal antibodies and identification of immunophenotypic profile, enhances the diagnostic accuracy and reproducibility.