Enterococci form an indigenous flora of the intestinal tract, oral cavity and the genitourinary tract of humans and animals. Once regarded as human commensals have emerged as significant nosocomial pathogens. They have high mortality rates, show intrinsic resistance to many antimicrobial agents, capable of transferring resistance genes to other bacteria hence emerge as leading clinical challenges for physicians. 225 Enterococcal isolates were obtained from various clinical samples. They were further differentiated to species level of which 203 (90.22%) isolates were identified as E.faecalis, 12 (5.33%) as E.faecium and 10 (4.44%) as E.durans. Isolates showed good sensitivity to Linezolid(100%) and Vancomycin(99.11%), E.faecalis(45.81%) and E. faecium(66.67%) had decreased sensitivity to Gentamicin. Treatment with combination therapy of aminoglycoside with a cell wall active agents has been recommended. Detection of resistance to aminoglycosides is of utmost important to prevent failure of combination therapy. Management of these infections with appropriate antibiotics yield desirable results