Aim: To evaluate effect on post endodontic pain after single sitting RCT in vital tooth following final irrigation with cold saline at 2–4 ˚C. Methodology: A total of 60 patients referred for intentional root canal treatment were selected for this study. The teeth were randomly divided into two groups. Group I– Control (n=30 normal saline) Group II– Cold Saline (n=30 at 2–4 ˚C) After routine chemo–mechanical preparation was completed teeth were finally irrigated over a period of 3 minutes using 10ml normal saline in Group I and cold saline at 2–4 ˚C in Group II. Pain levels were assessed by visual analog scale (VAS) after 6, 24, 48 hours. The data were then analyzed. Result: Group II final irrigant showed a significant reduction in postoperative pain levels in comparison to Group I (control group). Conclusion: Cryotherapy reduces postoperative pain following single–visit root canal treatment in teeth with vital pulps.