Background:Health care workers (HCWs) are at high risk of acquiring hepatitis B virus infection through occupational exposure which is preventable through Hepatitis B vaccination. Aims and Objectives:To study the response to HBV surface antigen vaccine by testing anti HBs titre among HCWs Material & Method: Blood samples of HCWs who have completed 3 doses of HBsAg vaccine was tested for anti HBs level. Result: Out of total 120 HCWs included in the study ,106 (88.33%) HCWs developed protective immunity (Above 10 mIU/ml) to HBV.68 (56.66%) HCWs showed titre between 10 mIU and 1000 mIU/ml and 38 (31.66%) showed titre above 1000 mIU/ml. 14.8% of males showed unprotective titre (Titre < 10mIU/ml) ,while (9.1%) of females showed unprotective titre(Titre < 10mIU/ml) .Non protective titre (<10mIU ) was found in 10 ( 19%) in the age group of > 35 years,followed by 8.3% below 30 years and 5.4% between 30–35 yrs Conclusion : Determining Anti HbsAg titre of HCWs after completion of 3 doses of Hepatitis B vaccination is essential for assessing the safety of HCWs