Agro industrial waste constitute valuable source for cultivation of microorganisms and production of enzyme. An attempt was made to study protease production at various temperatures by Fusarium oxysporum on agroindustrial waste such as dal mill waste, oil mill waste, molasses, fruit waste and vegetable garbage. Dal mill waste and vegetable garbage showed maximum protease production (12.7 µg /ml and 19.9 µg /ml respectively) was at 40 0C after 6 days of incubation. Oil mill waste and molasses however also produced maximum protease 19.7 µg /ml 13.9 µg/ml respectively) at 40 0C incubation temperature after 7 days of incubation. However fruit waste comparatively produced least amount of protease (4.5 µg /ml) at 500C after 5th days of incubation