Biochemical changes have been occurring in biological fluids and tissues of different types of malignancies. Most molecules found in blood and urine is found in saliva, but their concentrations were estimated to be one tenth to one thousandth of that in the blood. The present study aims to measure the levels of Lactate dehydrogenase & Alkaline Phosphatase enzymes and Immunoglobulins in saliva of Oral Squamous Cell Carcinoma (OSCC) patients, and apparantly healthy individuals as control group. Unstimulated (resting) saliva was collected from 30 newly diagnosed, untreated OSCC patients, in addition to 30 healthy individuals. According to the results of this study, saliva enzymes showed a significant increase in Lactate Dehydrogenase (LDH) and Alkaline Phosphatase (ALP) levels of OSCC in comparison to control group. Patients with OSCC resulted a significantly increase in saliva immunoglobulins and increase in saliva IgA was also noticed in comparison to control group. Saliva Immunoglobulins IgG and IgM were also higher in OSCC patients In conclusion, in a local malignancy like OSCC; the changes are more prominent in saliva.