Background: To assess the incidence and site of origin of Thyroidea ima artery and accessory thyroid arteries and their course in the neck region. Material & Method: The study was carried out on 104 cadavers of known age and sex. Thorough dissection was performed in neck region on both sides of the thyroid gland. Result: in present study, out of 104 cadavers, Thyroidea ima artery was found in one cadaver (incidence is 0.96%) that originated from Brachiocephalic trunk and in 5 cadavers accessory thyroid arteries arising from tracheal and esophageal arteries were found which were supplying medial and deep surface of thyroid gland. Conclusion: The information will be useful as reference for surgical procedures such as –tracheostomy, surgeries in suprasternal fossa, various types of lobectomy and others like catheterization and intervention radiology. It will be helpful to prevent complications such as profuse bleeding during surgical procedures.