Bruguiera gymnorhiza is one plant of mangroves that has not been studied as the anticancer potential. Previous research the methanolic extract of stem bark B. gymnorhiza is cytotoxic to HeLa of cancer cells against. The purpose of this research was done by the method fractionation methanol extract of liquid– solid partition with n–hexane solvent. Fractions were tested cytotoxic activity to HeLa cells by MTT method. The results showed that the fraction of n–hexane have cytotoxic activity with IC50 of 253.3 ug / ml and the identification showed the fatty acids and terpenoids which can be lethal cells by apoptosis.