Introduction: Infection control procedures are an integral part of modern dentistry and are continually evolving to meet the dental profession’s high standards. In absence of adequate infection control procedures there is realistic potential to transmit pathogenic microbes via endodontic instruments. Aim & Objectives : The aim of this article is to present practice trends among dental practitioners in central India towards sterilization and disinfection of endodontic instruments. The study was also intended to review the knowledge, attitude and practice of the dentist towards sterilization of endodontic instruments. Materials & method: A questionnaire containing 7 questions was distributed to private dental practitioners in central India. The questionnaire included questions about various methods of sterilization and disinfection available and utilized for endodontic instruments in routine dental practice. Result: It was seen that 42% dentists sterilize files in between appointments using chemical agents only, 12% used chemical as well as glass bead sterilizer while 0.04% used chemical method along with Autoclaving. Conclusion : Many of the dental practitioners were not following the basic protocols of the sterilization. There is an urgent need to increase the awareness amongst dentists regarding sterilization of the endodontic instruments as it can be highly contagious