Surgeons concern for complications and outcomes of gastrointestinal tract surgery, especially in patients who has received intraluminal contrast media before surgery. As of now in most of the diagnostic radiology, non ionic contrast agents have replaced barium sulphate as contrast medium of choice but still barium sulphate is being used as contrast medium at primary and secondary care centers. Therefore we conducted this experimental study to compare the effect of barium sulphate and gastrografin dye on intraluminal wound healing in the gastrointestinal tract of albino rats particularly in context of macroscopic and histological findings. Materials and Methods: This experimental study with prior approval of animal ethics committee was conducted in the department of general surgery at NSCB Medical College, Jabalpur, India. 45 Albino Rats were divided into 3 study groups (n= 15) and as per fixed protocol, linear incision was made on the anti mesenteric border of colon. O.5 ml of NS, Barium sulphate and gastrografin was instilled intraluminaly in Group A, B and C subsequently. Post instillation, colon was repaired transeversally with full thickness vicryl 6–0 sutures. Half of the rats from each group were sacrificed on POD 7 and rest on POD 21. After macroscopic examination, repaired area of bowel was taken out for histological assessment and the HPR were graded as per ‘tissue morphological score’ Shackleton KL et al [1]. Results: 50 % of survived rats in each group were sacrificed and analyzed as per tissue morphology score on POD 7. The tissue morphological score is based on neutrophillic infiltration, lymphocytic infiltration, collagen formation, granuloma formation and mucosal continuity. Mucosal continuity is a major determinant of wound healing and the best mucosal continuity was noted in–group C. About 90% cases of gastrografin dye group showed good mucosal continuity in comparison to 60 % cases of barium group. On POD 21, the findings of all the three groups were similar. Conclusion: Gastrografin (Non Ionic contrast) group showed good tissue healing in early phases with less complications. It is a better contrast medium as compared to barium sulphate. On the other hand, Barium sulphate resulted in more complications like adhesion and pus pocket formation.